Site-specific transgene insertion: an approach

dc.contributor	Wigley, P
dc.contributor	Becker, C
dc.contributor	Beltrame, J
dc.contributor	Blake, T
dc.contributor	Crocker, L
dc.contributor	Harrison, S
dc.contributor	Lyons, I
dc.contributor	McKenzie, Z
dc.contributor	Tearle, R
dc.contributor	Crawford, R
dc.contributor	et, al
dc.date.accessioned	2012-01-30T05:58:49Z
dc.date.available	2012-01-30T05:58:49Z
dc.date.issued	1994
dc.identifier.citation	Rep. Fert. Dev. (1994) 6(5): 585-588
dc.identifier.issn	1031-3613
dc.identifier.uri	http://livestocklibrary.com.au/handle/1234/15786
dc.description.abstract	Methods to improve the production of transgenic animals are being developed. Conventional transgenesis, involving microinjection of DNA into fertilized eggs, has a number of limitations. These result from the inability to control both the site of transgene insertion and the number of gene copies inserted. The approach described seeks to overcome these problems and to allow single copy insertion of transgenes into a defined site in animal genomes. The method involves the use of embryonic stem cells, gene targeting and the FLP recombinase system.
dc.publisher	CSIRO Publishing
dc.source.uri	http://www.publish.csiro.au/?act=view_file&file_id=RD9940585.pdf
dc.title	Site-specific transgene insertion: an approach
dc.type	Research
dc.description.version	Journal article
dc.identifier.volume	6
dc.identifier.page	585-588
dc.identifier.issue	5