Mouse testis Pdha-2 promoter upstream sequences confer tissue-and temporal-specific activity in transgenic mice

dc.contributor	Iannello, RC
dc.contributor	Young, JC
dc.contributor	Sumarsono, S
dc.contributor	Tymms, MJ
dc.contributor	Kola, I
dc.date.accessioned	2012-01-30T05:59:50Z
dc.date.available	2012-01-30T05:59:50Z
dc.date.issued	1994
dc.identifier.citation	Rep. Fert. Dev. (1994) 6(5): 599-604
dc.identifier.issn	1031-3613
dc.identifier.uri	http://livestocklibrary.com.au/handle/1234/15788
dc.description.abstract	Spermatogenesis is a complex process requiring the coordinate expression of a number of testis-specific genes. One of these, Pdha-2, codes for the murine testis-specific isoform of the E1 alpha subunit of the pyruvate dehydrogenase complex. To elucidate the mechanisms regulating its expression in vivo, we have begun to investigate the Pdha-2 promoter in transgenic mice. In this paper, a construct containing 3.0 kb of promoter and upstream sequences is reported to be sufficient for directing the testis-specific expression of a CAT reporter gene in mice harbouring the transgene. Similarly to the endogenous Pdha-2, the CAT gene is expressed in testis in a stage-specific manner. However, the 3.0-kb Pdha-2 promoter is not active in somatic tissue suggesting that repressor elements may be present within these sequences.
dc.publisher	CSIRO Publishing
dc.source.uri	http://www.publish.csiro.au/?act=view_file&file_id=RD9940599.pdf
dc.title	Mouse testis Pdha-2 promoter upstream sequences confer tissue-and temporal-specific activity in transgenic mice
dc.type	Research
dc.description.version	Journal article
dc.identifier.volume	6
dc.identifier.page	599-604
dc.identifier.issue	5