Abstract:
Activation rate, chromosome constituent and developmental pattern of porcineoocytes was examined in the presence and absence of cytochalasin B andcycloheximide following parthenogenetic stimulation. Treatment withcycloheximide after ethanol or Ca<emph type="7">2+ ionophore treatmentincreased the incidence of activation. The percentage of oocytes with two ormore female pronuclei was higher (P < 0· 05)in oocytes treated with cytochalasin B than in control orcycloheximide-treated oocytes. Treatment with both electrical stimulation andcytochalasin B increased the incidence of diploid chromosome spreads, andaccelerated development to the morula and blastocyst stage compared with thecontrol and cycloheximide-treated groups, suggesting a role of ploidy in thedevelopment of parthenote.