Abstract:
Spermatozoa collected with an artificial vagina (AV) were more resistant to cold shock as judged by motility and staining reaction than when ejaculated electrically (EE). Dilution of AV spermatozoa in AV seminal plasma caused some increase in susceptibility to cold shock, particularly as judged by motility scores, but the evidence suggests that EE seminal plasma renders spermatozoa more susceptible than does AV seminal plasma. Spermatozoa exposed to cold shock immediately after collection were most permeable to Congo red and became increasingly impermeable when incubated at 30