Net photosynthesis, water use efficiency, leaf water potential and leaf rolling as affected by water deficit in tropical upland rice

Abstract

Growth and production of tropical upland rice is often impeded by drought. Little is known on varietal response of CO2 assimilation to water deficit under tropical field conditions. A drought-susceptible semidwarf (IR20) and a drought-resistant traditional (Azucena) rice were grown in a dryland field experiment with sprinkler irrigation during the dry season in the Philippines. Differential irrigation was imposed for 11 days during vegetative growth using a line source sprinkler. Net photosynthesis, leaf conductance, transpiration, leaf rolling and leaf water potential were determined during the stress cycle at pre-noon and afternoon, with all measurements on the same leaf. No varietal differences in maximum photosynthetic rate and in the relationship between photosynthesis and leaf conductance were observed. In both rices, partial stomatal closure and nonstomatal inhibition reduced assimilation rates in the afternoon. Leaf water deficits restricted gas exchange through at least three apparently independent mechanisms: leaf rolling, reduced stomatal conductance and non-stomata1 inhibition which became evident only at severe degrees of stress. Stomata1 closure and leaf rolling were more sensitive to water deficit in Azucena which maintained higher leaf water potential throughout the stress cycle. Both stomatal closure and leaf rolling improved water use efficiency at moderate stress while nonstomatal inhibition of photosynthesis reduced water use efficiency.