Development of robust PCR-based DNA markers for each homoeo-allele of granule-bound starch synthase and their application in wheat breeding programs

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Development of robust PCR-based DNA markers for each homoeo-allele of granule-bound starch synthase and their application in wheat breeding programs

McLauchlan, A.; Ogbonnaya, F. C.; Hollingsworth, B.; Carter, M.; Gale, K. R.; Henry, R. J.; Holton, T. A.; Morell, M. K.; Rampling, L. R.; Sharp, P. J.; Shariflou, M. R.; Jones, M. G. K.; Appels, R.
URI: http://livestocklibrary.com.au/handle/1234/30809
Full Text: http://www.publish.csiro.au/?act=view_file&file_id=AR01036.pdf
Date: 2001

Abstract:

The absence of expression of the granule-bound starch synthase I (GBSSI) allele from chromosome 4A of wheat is associated with improved starch quality for making Udon noodles. Several PCR-based methods for the analysis of GBSS alleles have been developed for application in wheat. A widely applied approach has involved a simple PCR followed by electrophoretic separation of DNA products on agarose gels. The PCR amplifies one band from each of the loci on chromosomes 4A (

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